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KMID : 0043319970200050448
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Archives of Pharmacal Research
1997 Volume.20 No. 5 p.448 ~ p.453
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Protoplast fusion between Lentinula edodes and Coriolus versicolor
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Kim Chae-Kyun
Choi Eung-Chil
Kim Byung-Kak
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Abstract
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Protoplast fusion between isoleucine-, argihine- and thymidine-requiring auxotroph of Lentinula edodes and arginine-requiring auxotroph of Coriolus versicolor has been achieved using 30% polyethylene glycol (M.W.4000) in 10 mM -glycine solution (pH 8.0). Fusion hybrids were selected in the 0.6 M sucrose supplemented minimal media on the basis of nutritional complementation with fusion frequency of The hybrids included both parental and non-parental types in colony morphology, growth rate and isozyme patterns. We succeeded inter-order protoplast fusion between the auxotrophs of Lentinula edodes and Coriolus versicolor overcoming the natural barriers of incompatibility. We examined the characteristics of the hybrids and clarified the fusion rocess using electron microscopy.
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KEYWORD
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Lentinula edodes, Coriolus versicolor, Inter-order protoplast fusion, Isozyme pattern
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